nebnext ultra directional rna library prep kit Search Results


nebnext ultra ii directional rna library prep kit  (New England Biolabs)
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Nebnext Ultra Ii Directional Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra directional rna library  (New England Biolabs)
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Nebnext Ultra Directional Rna Library, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polya mrna workflow  (New England Biolabs)
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Polya Mrna Workflow, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polya + nebnext ultra directional rna library prep kit  (Illumina Inc)
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Polya + Nebnext Ultra Directional Rna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna library nebnext ultra directional rna library prep kit for  (Illumina Inc)
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Cdna Library Nebnext Ultra Directional Rna Library Prep Kit For, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra directional rna library prep kit with nebnext poly(a) mrna magnetic isolation module  (Illumina Inc)
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Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase <t>mRNA</t> levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).
Nebnext Ultra Directional Rna Library Prep Kit With Nebnext Poly(A) Mrna Magnetic Isolation Module, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultratm ii directional dual index rna library preparation kit  (StarSEQ GmbH)
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Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase <t>mRNA</t> levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).
Nebnext Ultratm Ii Directional Dual Index Rna Library Preparation Kit, supplied by StarSEQ GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra directional rna library prep kit for illumina with custom 8bp indexes  (Illumina Inc)
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Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase <t>mRNA</t> levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).
Nebnext Ultra Directional Rna Library Prep Kit For Illumina With Custom 8bp Indexes, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra directional rna library prep kit for illumina with custom 8bp indexes/product/Illumina Inc
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nebnext® ultratm directional rna library prep kit for illumina® 96 reactions  (Illumina Inc)
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Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase <t>mRNA</t> levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).
Nebnext® Ultratm Directional Rna Library Prep Kit For Illumina® 96 Reactions, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext® ultratm directional rna library prep kit for illumina® 96 reactions/product/Illumina Inc
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nebnext® ultratm directional rna library prep kit for illumina® 96 reactions - by Bioz Stars, 2026-02
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nebnext ultra directional rna library prep kit for illumina, v1.0  (Illumina Inc)
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Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase <t>mRNA</t> levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).
Nebnext Ultra Directional Rna Library Prep Kit For Illumina, V1.0, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra directional rna library prep kit for illumina, v1.0/product/Illumina Inc
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rrna-depleted rna by nebnext ultra directional rna library prep kit  (Illumina Inc)
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Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase <t>mRNA</t> levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).
Rrna Depleted Rna By Nebnext Ultra Directional Rna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna library nebnext® ultratm ii directional rna library prep kit  (Illumina Inc)
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Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase <t>mRNA</t> levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).
Cdna Library Nebnext® Ultratm Ii Directional Rna Library Prep Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase mRNA levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).

Journal: RNA

Article Title: tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner

doi: 10.1261/rna.066126.118

Figure Lengend Snippet: Endogenous targets are repressed upon tRF-3009 overexpresssion by tRNA-LeuTAA transfection. ( A ) Luciferase assay upon tRNA overexpression to produce tRF-3009. In parallel, Renilla luciferase mRNA levels were measured by qRT-PCR from the same cells on which luciferase assays were performed (normalized to Firefly mRNA levels) (*) P -value <0.005. ( B ) Cumulative distribution function (CDF) plots showing the repression of tRF-3009 targets upon overexpression of tRNA producing tRF-3009. Targets have been predicted using RNA22 algorithm . ( C ) Seed sequence complementarity in selected tRF-3009 targets that are identified in RNA-seq upon tRF-3009 expression. The number after 3′ UTR indicates the start position of the mRNA sequence match with 1 being the base immediately downstream from the stop codon. (Red line) Perfect base-pairing in seed; (black line) perfect base-pairing outside seed; (dashed line) wobble base-pairing. FER1 gene has two predicted complementary sites on its 3′ UTR. ( D ) Relative mRNA levels of indicated genes upon tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. (*) P -value <0.05; (**) P -value, 0.005 ( t -test). ( E ) Dual luciferase reporter assay on reporters containing the 3′ UTR of indicated genes after tRNA-LeuTAA transfection, leading to tRF-3009 overexpression. Perfect complementary sequence to the tRF-3009 serves as a positive control and all results are normalized to the “no site” reporter without any match to the tRF-3009 (*) P -value <0.05; (**) P -value, 0.005 ( t -test).

Article Snippet: Libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit with NEBNext Poly(A) mRNA Magnetic Isolation Module for Illumina.

Techniques: Transfection, Luciferase, Over Expression, Quantitative RT-PCR, Sequencing, RNA Sequencing, Expressing, Reporter Assay, Positive Control